Mouse Nuclear Factor Of Activated T-Cells Cytoplasmic 1 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Nuclear Factor Of Activated T-Cells, Cytoplasmic 1 in biofluid samples, such as tissue homogenates, cell lysates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . NFATC1 present in samples reacts with an anti-Mouse NFATC1 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-NFATC1 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active NFATC1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse NFATC1. Detection Target: Mouse Nuclear Factor Of Activated T-Cells, Cytoplasmic 1 Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.055ng/mL Storage Conditions: Store all components at 4┬░C This Mouse NFATC1 activity assay is for the quantitative determination of Nuclear Factor Of Activated T-Cells, Cytoplasmic 1 in samples including tissue homogenates, cell lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.